Journal: bioRxiv

Article Title: Genetic tuning of intrinsically photosensitive retinal ganglion cell subtype identity to drive visual behavior

doi: 10.1101/2024.04.25.590656

Figure Lengend Snippet: ( A ) Representative dendritic arbor tracing of M4 and M2 ipRGCs in control and Brn3bcKO mice. ( B ) Subtype identified by presence (M4) or absence (M2) of SMI-32 immunolabeling (dashed ellipses), examples are from Brn3bcKO cells. ( C ) (Top) Scheme of retrogradely labeling strategy of M1 ipRGCs. (Bottom) Representative dendritic arbor tracing of M1 ipRGCs in control and Brn3bcKO mice. ( D ) Sholl analysis of M4, M2 and M1 ipRGC subtypes (n = 5-36 cells/group). ipRGCs from Brn3bcKO mice present less complex dendritic arbors compared to control mice. ( E - F ) Brn3bcKO showed decreased dendritic field diameter (E) (n = 5-36 cells/group) and soma size (F) (n = 19-36 cells/group) in M4, M2 and M1 ipRGC subtypes than control mice. ( G ) Representative picture of M4 ipRGCs (dashed ellipses) filled with neurobiotin (NB) used for electrophysiological recordings. ( H ) Brn3bcKO M4 ipRGCs showed increased input resistance compared to control M4 ipRGCs (n = 6-8/group). ( I ) M4 ipRGC subtype excitability in control and Brn3bcKO (n =5-7 cells/group) retinas. Brn3bcKO M4 ipRGCs reached peak firing rate by 300pA and then showed marked depolarization block ( J ) Representative traces of photocurrent of M4 ipRGCs from control (black) and Brn3bcKO retinas. ( K ) Maximum and sustained phases of photocurrent in M4 ipRGCs (n = 4-6 cells/group). All data are Mean ± SEM, n.s. (not significant) P>0.05, *P<0.05, ***P<0.001, Student’s t- and Mann Whitney U tests and two-way repeated measures ANOVA with Tukey’s multiple comparisons test.

Article Snippet: All recordings were made with internal solution containing 125 mM K-gluconate, 2 mM CaCl 2 , 2 mM MgCl 2 , 10 mM EGTA, 10 mM HEPES, 10 mM Na 2 -ATP, 0.5 mM Na-GTP, and 0.3% Neurobiotin (Vector Laboratories).

Techniques: Control, Immunolabeling, Labeling, Blocking Assay, MANN-WHITNEY

Journal: Endocrinology

Article Title: Adiponectin Exerts Neurotrophic Effects on Dendritic Arborization, Spinogenesis, and Neurogenesis of the Dentate Gyrus of Male Mice

doi: 10.1210/en.2015-2078

Figure Lengend Snippet: Antibody Table

Article Snippet: Next day, the sections were coincubated with the following Alexa Fluor-conjugated secondary antibodies for 4 hours: antirabbit Alexa Fluor 647 goat IgG, antimouse Alexa Fluor 594 goat IgG, and antirat Alexa Fluor 488 goal IgG (1:400; Life Technologies) ( ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Peptide/Protein Target Antigen Sequence (if Known) Name of Antibody Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody Species Raised in; Monoclonal or Polyclonal Dilution Used Ki67 Synthetic peptide conjugated to KLH derived from within residues 1200 − 1300 of Human Ki67 Anti-Ki67 antibody Abcam, Ab15580 Rabbit; polyclonal 1:1000 Doublecortin (DCX) epitope mapping at the C-terminus of Doublecortin of human origin Doublecortin antibody (C-18) Santa Cruz Biotechnology, Inc, sc-8066 Goat; polyclonal 1:200 BrdU Anti-BrdU antibody [BU1/75 (ICR1)] Accurate Chemical, YSRTMCA2060GA Rat; monoclonal 1:400 NeuN Anti-NeuN antibody, clone A60 Millipore, MAB377 Mouse; monoclonal 1:1000 GFAP Anti-glial fibrillary acidic protein (GFAP) antibody Millipore, AB5804 Rabbit; polyclonal 1:1000 Biotinylated antirabbit IgG Biotinylated goat antirabbit IgG antibody Vector Laboratories, BA-1000 Goat 1:400 Biotinylated antigoat IgG Biotinylated horse antigoat IgG antibody Vector Laboratories, BA-9500 Horse 1:400 Alexa Fluor 488 goat antirat IgG Goat antirat IgG (H + L) secondary antibody, Alexa Fluor 488 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A11006","term_id":"492389","term_text":"A11006"}} A11006 Goat 1:400 Alexa Fluor 546 goat antimouse IgG F(ab')2-goat antimouse IgG (H + L) secondary antibody, Alexa Fluor 546 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A11018","term_id":"492393","term_text":"A11018"}} A11018 Goat 1:400 Alexa Fluor 647 goat antirabbit IgG F(ab')2-goat antirabbit IgG (H + L) secondary antibody, Alexa Fluor 647 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A21246","term_id":"641368","term_text":"A21246"}} A21246 Goat 1:400 Open in a separate window Abbreviation: H+L, Heavy and light chain.

Techniques: Sequencing, Derivative Assay, Plasmid Preparation